Workshop: CRISPR for NeuroGrads

 – how do I actually do? And when should I use siRNA instead?

By Søren Høgh from GE Healthcare

Part 1 14.00-14:50

Title: Fundamentals of CRISPR-Cas9 Gene Editing  

Brief summary: CRISPR-Cas9 is an exciting tool for gene editing that is becoming increasingly popular. We will introduce the elementary concepts of how CRISPR-Cas9 gene editing works, the basic reagents needed, and the basic workflow of a gene editing experiment using CRISPR-Cas9 products. This is a presentation geared towards providing information to the researcher that does not yet consider themselves an expert in this technology. In addition, we will highlight some of the primary applications for which this technology is currently being used.  

COFFEE BREAK

Part 2 15.00-17.00

A) Title: Considerations for a CRISPR-Cas9 experiment  

Brief summary: There are multiple decisions to be made when setting up and optimal gene editing experiment. We will look at considerations for designing a gene editing experiment including how to choose which Cas9 format is best for your experiment, and selecting between a crRNA and a sgRNA approach. We will discuss and review the workflows for common experimental approaches. Lastly, we will touch on the “dos and don’ts” to consider when designing and troubleshooting your experiments.  

B) Title: A comparison of RNAi and gene editing  

Brief summary: We will review some of the considerations in choosing between using gene editing or RNAi in your experiments. For an RNAi experimental approach, we will review reagents available for gene silencing applications covering our primary siRNA and shRNA tools and how to choose which ones will best meet your experimental needs.  We will introduce Accell siRNA, which is ideal for performing siRNA in difficult to transfect cells such as neurons.  

PIZZA AND BEER for NeuroGrads

You can join the event on Facebook at https://www.facebook.com/events/1698527663805489/